Selective Isolation of Bacillus sphaericus from Soil by Use of Acetate as the Only Major Source of Carbon.
نویسندگان
چکیده
A simple chemically defined medium containing sodium acetate (37 or 74 mM) as the only major source of carbon was inoculated with soil from various locations. The bacteria which grew most rapidly at 30 degrees C were almost entirely strains of Bacillus sphaericus and Arthrobacter species. Pasteurization of the soils made the medium selective for B. sphaericus. Several new strains were isolated, and the peptidoglycans of their cell walls were examined.
منابع مشابه
Selective Process for Efficient Isolation of Soil Bacillus spp.
We were able to isolate Bacillus thuringiensis from environmental samples with a background of 10 bacteria per g of soil. Our selection process differed significantly from classical selection methods which permit only the desired organism to grow. In our process, germination of B. thuringiensis spores was selectively inhibited by sodium acetate, while most of the undesired sporeformers germinat...
متن کاملDetermination of anthrax foci through isolation of Bacillus anthracis form soil samples of different regions of Iran
To isolate and detect anthrax spores form soil in different regions of Iran in order to find the anthrax foci‚ a total of 668 environmental specimens were collected during 2003-2004. Bacterial endospores were extracted from soil specimens via flotation in distilled water, incubation at room temperature, filtration, heat shock and culture on blood agar and selective PLET media. Bacillus anthraci...
متن کاملIsolation of Anthrax Spores from Soil in Endemic Regions of Isfahan, Iran
To isolate and detect anthrax spores from soil in different regions of Isfahan, Iran a total of 60 environmental specimens were collected during 2003. Bacterial endospores were extracted via flotation in distilled water and were cultured on blood agar and selective PLET media. Bacillus anthracis was identified using bacteriological and biological tests. Viable Bacillus anthracis spores were iso...
متن کاملجداسازی باکتریهای دارای آنزیم ارگانوفسفوروس اسید انهیدراز و شناسایی سویه دارای بیشترین فعالیت هیدرولازی
Background and purpose: In Iran, Organ phosphorus pesticides such as chloropyrifos and diazinon are widely used in agriculture. These compounds inhibit activity of cholinesterase in nearly irreversible manner resulting in malfunction of nerve impulse transmission. This result in humans can produce illness or even death. Therefore, the present study aims to isolate various bacterial strains...
متن کاملIsolation and Partial Characterization of a Bacterial Thermostable Polymethyl Galacturonase from a Newly Isolated Bacillus sp. strain BR1390
Background: Pectinases are pectin degrading class of enzymes including polygalacturonase (PG), polymethyl galacturonase (PMG), pectate lyase (PEL), and pectin esterase (PE) that are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juices. Objectives: A highly methylated pectin degrading bacterium from soil covered with fruit wa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 49 6 شماره
صفحات -
تاریخ انتشار 1985